M2 macrophage-derived exosomal microRNAs inhibit cell migration and invasion in gliomas through PI3K/AKT/mTOR signaling pathway

Abstract Background Glioma, the most common primary brain tumor, account Preparing figures for 30 to 40% of all intracranial tumors. Herein, we aimed study effects M2 macrophage-derived exosomal microRNAs (miRNAs) on glioma cells. Methods First, identified seven differentially expressed miRNAs in infiltrating macrophages and detected expression these macrophages. We then selected hsa-miR-15a-5p (miR-15a) hsa-miR-92a-3p (miR-92a) follow-up studies, confirmed that miR-15a miR-92a were under-expressed macrophage exosomes. Subsequently, demonstrated exosomes promoted migration invasion cells, while had opposite Next, performed target gene prediction four databases conducted validation by qRT-PCR, western blot dual luciferase reporter assays. Results The results revealed bound CCND1 RAP1B, respectively. Western assays interference with or RAP1B reduced phosphorylation level AKT mTOR, indicating both can activate PI3K/AKT/mTOR signaling pathway. Conclusion Collectively, findings indicate inhibit cell cells through